Tetsuo Mizutori was born in Tokyo on December 25, 1938. In addition to being a voice actor, Mizutori was also a lecturer in the Katsuta Voice Actor's Academy. He has been represented by the Japanese talent agency Arts Vision Incorporated.

Mizutori began his career in anime voice acting in the 1970s. During that decade, his voice was heard in Dokonjo Gaeru (as Mogura), Kashi no Ki Mokku, Umi no Triton, Doraemon, Dororon Enma-kun (as Madoromin), Jungle Kurobe (as Pao Pao), Kerokko Demetan, Shinzo Ningen Casshan, Alps no Shojo Heidi, Hurricane Polymar, Urikupen Kyujotai (as Wolf), Maya the Bee, Time Bokan, Machine Hayabusa (as Iwada Tetsuji), Robokko Beeton (as Gakiranger), Angie Girl (as Alfred), Kyoryu Daiseno Aizenborg (as Doctor Torii), Lupin III: Part II (as Rihei Tadanori), Conan, the Boy in Future (as Kuzo and Luca), Kagaku Ninja-Tai Gatchaman F, and in Mobile Suit Gundam (as Gadem).

Mizutori's voice acting career remained strong during the 1980s, when he starred in a number of productions produced by Studio Pierrot. During the 1980s, his voice could be heard in Tomorrow's Joe 2 (as Tono), Densetsu Kyojin Ideon (as Frendali Limiter), The Wonderful Adventures of Nils (as Ondori), Dr. Slump, Meiken Jolie, Rokushin Gattai God Mars (as Beta), Urusei Yatsura, Wanwan Sanjushi, Gyakuten Ippatsu-man, Captain Tsubasa, the Kinnikuman franchise (as Brocken Jr., Yosaku-san, Brockenman, Franky, Kamata, and Wild Bakuto), Serendipity Monogatari: Pyua-tou no Nakama-tachi, Chikkun Takkun (as Chikkun’s Father), Nausicaa of the Valley of the Wind (as Commando A), Aoki Ryusei SPT Layzner (as Chifurenko), Hey! Bunbuu, Maison Ikkoku (as Takeshi), Super Mario Brothers: Peach-hime Kyuushutsu Daisakusen (as Nokonoko), Uchuusen Sagittarius, Urban Square – Kouhaku no Tsuigeki, Anime Sanjushi, City Hunter (as Sanchesu), Esper Mami, Fist of the North Star 2 (as Geira), The Laughing Target, Anpanman (as Tekkotsu Hollerman Blue), Osomatsu-kun (as Beshi and Father), Ginga Eiyu Densetsu (as Bronze), Ahiru no Quack, Aoi Blink, and Dragon Ball Z (as Zald).

Mizutori's anime voice acting career slowed down during the 1990s and 2000s, but his voice could still occasionally be heard. The credits from his later years include Heisei Tensai Bakabon, Mashin Hero Wataru (as Touch Down), Dark Warrior (as Rudy Bochwitz), Cooking Papa (as Osamu Fukai), Nintama Rantaro, Konpeki no Kantai (as Naohiko Kumagai), R.O.D. –The TV-, Stratos 4, and Slayers: Evolution-R.

In addition to anime voice acting, Mizutori provided voices for video games. His video game credits include the Gundam series, Mirai Shonen Conan, the Justice Gakuen series, Namco x Capcom, Shinobi, and Summon Night. Mizutori also provided his voice for some Japanese dubs of foreign productions. These credits include A Bridge Too Far, The Dirty Dozen, Major League, and Thunderbirds.

Sadly, Tetsuo Mizutori passed away on July 14, 2010 at the age of 71.